› 5vwi

X-ray diffraction
1.75Å resolution

Crystal structure of human Scribble PDZ1:Beta-PIX complex

Released:
DOI: 10.2210/pdb5vwi/pdb
PDB entry 5vwi coloured by chain, front view.
PDB entry 5vwi coloured by chain, side view.
PDB entry 5vwi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the differential interaction of Scribble PDZ domains with the guanine nucleotide exchange factor β-PIX.
Lim KYB, Gödde NJ, Humbert PO, Kvansakul M
J Biol Chem 292 20425-20436 (2017)
PMID: 29061852

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166232 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein scribble homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q14160 (Residues: 1002-1092; Coverage: 6%)
Gene names: CRIB1, KIAA0147, LAP4, SCRB1, SCRIB, VARTUL
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
beta-PIX Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 945 Da
Source organism: Homo sapiens
Expression system: Escherichia coli K-12

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 37.869Å b: 44.163Å c: 60.465Å
α: 90° β: 93.61° γ: 90°
R-values:
R R work R free
0.198 0.194 0.236
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation.
Bonello et al. (2019)
2 more

4 mentions without citation

PDZ Domains as Drug Targets.
Christensen et al. (2019)
3 more