› 5vwk

X-ray diffraction
2.35Å resolution

Crystal structure of human Scribble PDZ1:Beta-PIX complex

Released:
DOI: 10.2210/pdb5vwk/pdb
PDB entry 5vwk coloured by chain, front view.
PDB entry 5vwk coloured by chain, side view.
PDB entry 5vwk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the differential interaction of Scribble PDZ domains with the guanine nucleotide exchange factor β-PIX.
Lim KYB, Gödde NJ, Humbert PO, Kvansakul M
J Biol Chem 292 20425-20436 (2017)
PMID: 29061852

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein scribble homolog Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q14160 (Residues: 700-816; Coverage: 7%)
Gene names: CRIB1, KIAA0147, LAP4, SCRB1, SCRIB, VARTUL
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Beta-PIX Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 8 amino acids
Theoretical weight: 945 Da
Source organism: Homo sapiens
Expression system: Escherichia coli K-12

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P43212
Unit cell:
a: 74.76Å b: 74.76Å c: 222.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.256
Expression system: Escherichia coli K-12

Quick links

Citations

2 review citations

Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation.
Bonello et al. (2019)
1 more

6 mentions without citation

PDZ Domains as Drug Targets.
Christensen et al. (2019)
5 more