› 5vwv

X-ray diffraction
1.9Å resolution

Bak core latch dimer in complex with Bim-BH3 - Cubic

Released:
DOI: 10.2210/pdb5vwv/pdb
PDB entry 5vwv coloured by chain, front view.
PDB entry 5vwv coloured by chain, side view.
PDB entry 5vwv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design.
Brouwer JM, Lan P, Cowan AD, Bernardini JP, Birkinshaw RW, van Delft MF, Sleebs BE, Robin AY, Wardak A, Tan IK, Reljic B, Lee EF, Fairlie WD, Call MJ, Smith BJ, Dewson G, Lessene G, Colman PM, Czabotar PE
Mol Cell 68 659-672.e9 (2017)
PMID: 29149594

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128966 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2 homologous antagonist/killer Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16611 (Residues: 23-186; Coverage: 78%)
Gene names: BAK, BAK1, BCL2L7, CDN1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Bcl-2-like protein 11 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 3.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43521 (Residues: 141-165; Coverage: 13%)
Gene names: BCL2L11, BIM
Sequence domains: Bcl-x interacting, BH3 domain

Ligands and Environments

4 bound ligands:
Ligand TFA 1 x TFA
Ligand EDO 6 x EDO
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P4132
Unit cell:
a: 139.404Å b: 139.404Å c: 139.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.177
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Mitochondria as multifaceted regulators of cell death.
Bock et al. (2020)
11 more

3 mentions without citation

Structural basis of BAK activation in mitochondrial apoptosis initiation.
Singh et al. (2022)
2 more