› 5vwx

X-ray diffraction
2.49Å resolution

Bak core latch dimer in complex with Bim-h0-h3Glt

Released:
DOI: 10.2210/pdb5vwx/pdb
PDB entry 5vwx coloured by chain, front view.
PDB entry 5vwx coloured by chain, side view.
PDB entry 5vwx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design.
Brouwer JM, Lan P, Cowan AD, Bernardini JP, Birkinshaw RW, van Delft MF, Sleebs BE, Robin AY, Wardak A, Tan IK, Reljic B, Lee EF, Fairlie WD, Call MJ, Smith BJ, Dewson G, Lessene G, Colman PM, Czabotar PE
Mol Cell 68 659-672.e9 (2017)
PMID: 29149594

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2 homologous antagonist/killer Chains: A, C
Molecule details ›
Chains: A, C
Length: 170 amino acids
Theoretical weight: 19.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16611 (Residues: 23-186; Coverage: 78%)
Gene names: BAK, BAK1, BCL2L7, CDN1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Bcl-2-like protein 11 Chains: B, D
Molecule details ›
Chains: B, D
Length: 23 amino acids
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43521 (Residues: 142-164; Coverage: 12%)
Gene names: BCL2L11, BIM
Sequence domains: Bcl-x interacting, BH3 domain

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 61.629Å b: 76.481Å c: 77.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.25 0.305
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Mitochondria as multifaceted regulators of cell death.
Bock et al. (2020)
11 more

1 mention without citation

Structure of the BAK-activating antibody 7D10 bound to BAK reveals an unexpected role for the α1-α2 loop in BAK activation.
Robin et al. (2022)