5vyb

X-ray diffraction
2.4Å resolution

Structure of the carbohydrate recognition domain of Dectin-2 complexed with a mammalian-type high mannose Man9GlcNAc2 oligosaccharide

Released:
Source organism: Homo sapiens
Primary publication:
Mechanism of pathogen recognition by human dectin-2.
J Biol Chem 292 13402-13414 (2017)
PMID: 28652405

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-179578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
C-type lectin domain family 6 member A Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6EIG7 (Residues: 64-209; Coverage: 70%)
Gene names: CLEC6A, CLECSF10, DECTIN2
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, MAN
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 64.055Å b: 78.834Å c: 76.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.24
Expression system: Escherichia coli BL21(DE3)