› 5w0h

X-ray diffraction
1.11Å resolution

Structure of U2AF65 (U2AF2) RRM2 at 1.11 Angstrom Resolution

Released:
DOI: 10.2210/pdb5w0h/pdb
PDB entry 5w0h coloured by chain, front view.
PDB entry 5w0h coloured by chain, side view.
PDB entry 5w0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-Associated Mutations Mapped on High-Resolution Structures of the U2AF2 RNA Recognition Motifs.
Glasser E, Agrawal AA, Jenkins JL, Kielkopf CL
Biochemistry 56 4757-4761 (2017)
PMID: 28850223

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 8.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 258-336; Coverage: 17%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 33.43Å b: 38.203Å c: 72.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.168
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Somatic Mutations in Core Spliceosome Components Promote Tumorigenesis and Generate an Exploitable Vulnerability in Human Cancer.
Sette et al. (2022)
1 more

1 mention without citation

Cancer-Associated Mutations Mapped on High-Resolution Structures of the U2AF2 RNA Recognition Motifs.
Glasser et al. (2017)