› 5w0v

X-ray diffraction
2.82Å resolution

Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34

Released:
DOI: 10.2210/pdb5w0v/pdb
PDB entry 5w0v coloured by chain, front view.
PDB entry 5w0v coloured by chain, side view.
PDB entry 5w0v coloured by chain, top view.
Source organism: Kluyveromyces lactis
Primary publication:
Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123.
An S, Yoon J, Kim H, Song JJ, Cho US
Elife 6 (2017)
PMID: 29035199

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135461 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 1116 amino acids
Theoretical weight: 122.97 KDa
Source organism: Kluyveromyces lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CMF0 (Residues: 1-1113; Coverage: 100%)
Gene name: KLLA0_E20769g
Sequence domains:
Histone H4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 34 amino acids
Theoretical weight: 3.54 KDa
Source organism: Kluyveromyces lactis
Expression system: Not provided
UniProt:
  • Canonical: P02309 (Residues: 2-35; Coverage: 33%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 78.793Å b: 87.697Å c: 101.623Å
α: 79.59° β: 81.45° γ: 71.7°
R-values:
R R work R free
0.172 0.17 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

H3-H4 Histone Chaperone Pathways.
Grover et al. (2018)
3 more

1 mention without citation

Nuclear import of histones.
Bernardes et al. (2020)