5w1v

X-ray diffraction
3.31Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-210559 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, F, K, P
Molecule details ›
Chains: A, F, K, P
Length: 278 amino acids
Theoretical weight: 32.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P13747 (Residues: 22-299; Coverage: 83%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G, L, Q
Molecule details ›
Chains: B, G, L, Q
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein UL40 Chains: C, H, M, R
Molecule details ›
Chains: C, H, M, R
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human herpesvirus 5 strain AD169
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P16780 (Residues: 15-23; Coverage: 4%)
Gene name: UL40
GF4 T cell receptor alpha chain Chains: D, I, N, S
Molecule details ›
Chains: D, I, N, S
Length: 207 amino acids
Theoretical weight: 22.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins
GF4 T cell receptor beta chain Chains: E, J, O, T
Molecule details ›
Chains: E, J, O, T
Length: 246 amino acids
Theoretical weight: 27.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 71.646Å b: 228.246Å c: 276.872Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.268
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'