› 5w38

X-ray diffraction
1.8Å resolution

1.80A resolution structure of human IgG3 Fc (N392K)

Released:
DOI: 10.2210/pdb5w38/pdb
PDB entry 5w38 coloured by chain, front view.
PDB entry 5w38 coloured by chain, side view.
PDB entry 5w38 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of the Man5 glycoform of human IgG3 Fc.
Shah IS, Lovell S, Mehzabeen N, Battaile KP, Tolbert TJ
Mol Immunol 92 28-37 (2017)
PMID: 29031045

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Immunoglobulin heavy constant gamma 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 25.22 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P01860 (Residues: 155-375; Coverage: 50%)
Gene name: IGHG3
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 64.429Å b: 60.443Å c: 71.396Å
α: 90° β: 109.24° γ: 90°
R-values:
R R work R free
0.186 0.184 0.223
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

A synopsis of recent developments defining how N-glycosylation impacts immunoglobulin G structure and function.
Yamaguchi et al. (2020)
1 more

7 mentions without citation

A stable engineered human IgG3 antibody with decreased aggregation during antibody expression and low pH stress.
Saito et al. (2019)
6 more