› 5w4g

X-ray diffraction
2.04Å resolution

Importin binding to NLS peptide of DNA polymerase lambda

Released:
DOI: 10.2210/pdb5w4g/pdb
PDB entry 5w4g coloured by chain, front view.
PDB entry 5w4g coloured by chain, side view.
PDB entry 5w4g coloured by chain, top view.
Source organisms:
Entry authors: Pedersen LC, London RE

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156538 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
DNA polymerase lambda Chain: A
Molecule details ›
Chain: A
Length: 29 amino acids
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UGP5 (Residues: 4-32; Coverage: 5%)
Gene name: POLL

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 78.195Å b: 89.747Å c: 98.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.2
Expression systems:
  • Escherichia coli
  • Not provided

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