› 5w5z

X-ray diffraction
2Å resolution

Crystal structure of BAXP168G in complex with an activating antibody at high resolution

Released:
DOI: 10.2210/pdb5w5z/pdb
PDB entry 5w5z coloured by chain, front view.
PDB entry 5w5z coloured by chain, side view.
PDB entry 5w5z coloured by chain, top view.
Source organisms:
Primary publication:
Ensemble Properties of Bax Determine Its Function.
Robin AY, Iyer S, Birkinshaw RW, Sandow J, Wardak A, Luo CS, Shi M, Webb AI, Czabotar PE, Kluck RM, Colman PM
Structure 26 1346-1359.e5 (2018)
PMID: 30122452

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231355 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
3C10 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.46 KDa
Source organism: Rattus norvegicus
Structure domains: Immunoglobulins
3C10 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 24.92 KDa
Source organism: Rattus norvegicus
Apoptosis regulator BAX Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07812 (Residues: 32-192; Coverage: 84%)
Gene names: BAX, BCL2L4
Sequence domains: Apoptosis regulator proteins, Bcl-2 family

Ligands and Environments

4 bound ligands:
Ligand EDO 3 x EDO
Ligand PG4 2 x PG4
Ligand PGE 2 x PGE
Ligand MES 1 x MES
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P41212
Unit cell:
a: 67.863Å b: 67.863Å c: 264.243Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.268
Expression system: Escherichia coli

Quick links

Citations

4 review citations

BAX, BAK, and BOK: A Coming of Age for the BCL-2 Family Effector Proteins.
Moldoveanu et al. (2020)
3 more

4 mentions without citation

Physiological and pharmacological modulation of BAX.
Spitz et al. (2022)
3 more