› 5w8k

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 332 amino acids
Theoretical weight: 36.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 1-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 4 x NAI
3 bound ligands:
Ligand 9Y7 3 x 9Y7
Ligand MLA 5 x MLA
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 167.76Å b: 81.08Å c: 120.44Å
α: 90° β: 117.14° γ: 90°
R-values:
R R work R free
0.139 0.138 0.161
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Targeting cancer metabolism in the era of precision oncology.
Stine et al. (2022)
12 more

2 mentions without citation

Discovery and Optimization of Potent, Cell-Active Pyrazole-Based Inhibitors of Lactate Dehydrogenase (LDH).
Rai et al. (2017)
1 more