› 5wbl

X-ray diffraction
3.35Å resolution

Crystal structure of the Arabidopsis thaliana Raptor in complex with the TOS peptide of human PRAS40

Released:
DOI: 10.2210/pdb5wbl/pdb
PDB entry 5wbl coloured by chain, front view.
PDB entry 5wbl coloured by chain, side view.
PDB entry 5wbl coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Yang H, Jiang X, Li B, Yang HJ, Miller M, Yang A, Dhar A, Pavletich NP
Nature 552 368-373 (2017)
PMID: 29236692

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188163 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulatory-associated protein of TOR 1 Chain: A
Molecule details ›
Chain: A
Length: 1287 amino acids
Theoretical weight: 141.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q93YQ1 (Residues: 1-1344; Coverage: 96%)
Gene names: At3g08850, RAPTOR1, RAPTOR1B, T16O11.22
Sequence domains:
Proline-rich AKT1 substrate 1 Chain: T
Molecule details ›
Chain: T
Length: 16 amino acids
Theoretical weight: 1.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96B36 (Residues: 124-139; Coverage: 6%)
Gene names: AKT1S1, PRAS40

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 89.1Å b: 113.1Å c: 151.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.266
Expression systems:
  • Insect cell expression vector pTIE1
  • Not provided

Quick links

Citations

86 review citations

mTOR at the nexus of nutrition, growth, ageing and disease.
Liu et al. (2020)
85 more

7 mentions without citation

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Yang et al. (2017)
6 more