5wcu

X-ray diffraction
5.53Å resolution

Crystal structure of 167 bp nucleosome bound to the globular domain of linker histone H5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-119342 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H2A Chains: C, G, M, Q
Histone H5 Chains: U, V
Molecule details ›
Chains: U, V
Length: 76 amino acids
Theoretical weight: 8.2 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02259 (Residues: 23-98; Coverage: 40%)
Sequence domains: linker histone H1 and H5 family
DNA (167-MER) Chains: I, S
Molecule details ›
Chains: I, S
Length: 167 nucleotides
Theoretical weight: 51.32 KDa
DNA (167-MER) Chains: J, T
Molecule details ›
Chains: J, T
Length: 167 nucleotides
Theoretical weight: 51.79 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 65.926Å b: 108.543Å c: 180.77Å
α: 100.79° β: 90.08° γ: 89.94°
R-values:
R R work R free
0.195 0.189 0.238
Expression system: Escherichia coli