› 5wdm

X-ray diffraction
2.8Å resolution

An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein

Released:
DOI: 10.2210/pdb5wdm/pdb
PDB entry 5wdm coloured by chain, front view.
PDB entry 5wdm coloured by chain, side view.
PDB entry 5wdm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein.
Glidden MD, Aldabbagh K, Phillips NB, Carr K, Chen YS, Whittaker J, Phillips M, Wickramasinghe NP, Rege N, Swain M, Peng Y, Yang Y, Lawrence MC, Yee VC, Ismail-Beigi F, Weiss MA
J Biol Chem 293 47-68 (2018)
PMID: 29114035

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin B chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 57 amino acids
Theoretical weight: 6.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-110; Coverage: 66%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 43.382Å b: 85.771Å c: 45.696Å
α: 90° β: 110.89° γ: 90°
R-values:
R R work R free
0.257 0.251 0.314
Expression system: Not provided

Quick links

Citations

3 review citations

A thing of beauty: Structure and function of insulin's "aromatic triplet".
Weiss et al. (2018)
2 more

1 mention without citation

An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein.
Glidden et al. (2018)