› 5wdp

X-ray diffraction
1.35Å resolution

H-Ras mutant L120A bound to GMP-PNP at 277K

Released:
DOI: 10.2210/pdb5wdp/pdb
PDB entry 5wdp coloured by chain, front view.
PDB entry 5wdp coloured by chain, side view.
PDB entry 5wdp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deconstruction of the Ras switching cycle through saturation mutagenesis.
Bandaru P, Shah NH, Bhattacharyya M, Barton JP, Kondo Y, Cofsky JC, Gee CL, Chakraborty AK, Kortemme T, Ranganathan R, Kuriyan J
Elife 6 (2017)
PMID: 28686159

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GNP 1 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: R32
Unit cell:
a: 89.988Å b: 89.988Å c: 135.969Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.154
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Oncogenic Ras Isoforms Signaling Specificity at the Membrane.
Nussinov et al. (2018)
11 more

2 mentions without citation

Deconstruction of the Ras switching cycle through saturation mutagenesis.
Bandaru et al. (2017)
1 more