› 5wea

X-ray diffraction
3.12Å resolution

Human Argonaute2 Helix-7 Mutant

Released:
DOI: 10.2210/pdb5wea/pdb
PDB entry 5wea coloured by chain, front view.
PDB entry 5wea coloured by chain, side view.
PDB entry 5wea coloured by chain, top view.
Source organisms:
Primary publication:
Helix-7 in Argonaute2 shapes the microRNA seed region for rapid target recognition.
Klum SM, Chandradoss SD, Schirle NT, Joo C, MacRae IJ
EMBO J 37 75-88 (2018)
PMID: 28939659

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193884 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 97.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IPH 1 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 63.261Å b: 107.88Å c: 68.723Å
α: 90° β: 107.46° γ: 90°
R-values:
R R work R free
0.254 0.252 0.282
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

10 review citations

Metazoan MicroRNAs.
Bartel DP. (2018)
9 more

2 mentions without citation

Helix-7 in Argonaute2 shapes the microRNA seed region for rapid target recognition.
Klum et al. (2018)
1 more