› 5wh1

X-ray diffraction
3.39Å resolution

Apo form of the C-terminal region of human Transcription Factor IIB

Released:
DOI: 10.2210/pdb5wh1/pdb
PDB entry 5wh1 coloured by chain, front view.
PDB entry 5wh1 coloured by chain, side view.
PDB entry 5wh1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator cross-talk.
Bratkowski M, Unarta IC, Zhu L, Shubbar M, Huang X, Liu X
J Biol Chem 293 1651-1665 (2018)
PMID: 29158257

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor IIB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00403 (Residues: 107-316; Coverage: 67%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: Transcription factor TFIIB repeat

Ligands and Environments

1 bound ligand:
Ligand SO4 26 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 108.165Å b: 126.461Å c: 158.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Terminus-Associated Non-coding RNAs: Trash or Treasure?
Ni et al. (2020)
1 more

2 mentions without citation

Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator cross-talk.
Bratkowski et al. (2018)
1 more