› 5whk

X-ray diffraction
2.5Å resolution

Crystal structure of Fab fragment of antibody DX-2507 bound to FcRn-B2M

Released:
DOI: 10.2210/pdb5whk/pdb
PDB entry 5whk coloured by chain, front view.
PDB entry 5whk coloured by chain, side view.
PDB entry 5whk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody.
Kenniston JA, Taylor BM, Conley GP, Cosic J, Kopacz KJ, Lindberg AP, Comeau SR, Atkins K, Bullen J, TenHoor C, Adelman BA, Sexton DJ, Edwards TE, Nixon AE
J Biol Chem 292 17449-17460 (2017)
PMID: 28878017

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-157344 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 22.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: S6BAN1 (Residues: 20-246; Coverage: 73%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 22.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NS95 (Residues: 22-43, 44-48, 49-234; Coverage: 98%)
Gene name: IGL@
Sequence domains:
Structure domains: Immunoglobulins
IgG receptor FcRn large subunit p51 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P55899 (Residues: 1-297; Coverage: 80%)
Gene names: FCGRT, FCRN
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61769 (Residues: 1-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand NHE 2 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 59.56Å b: 70.45Å c: 256.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.225
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Targeting FcRn to Generate Antibody-Based Therapeutics.
Ward et al. (2018)
3 more

5 mentions without citation

Improving Antibody Therapeutics by Manipulating the Fc Domain: Immunological and Structural Considerations.
Delidakis et al. (2022)
4 more