› 5wi2

X-ray diffraction
2.5Å resolution

Crystal structure of the KA1 domain from human Chk1

Released:
DOI: 10.2210/pdb5wi2/pdb
PDB entry 5wi2 coloured by chain, front view.
PDB entry 5wi2 coloured by chain, side view.
PDB entry 5wi2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intramolecular autoinhibition of checkpoint kinase 1 is mediated by conserved basic motifs of the C-terminal kinase-associated 1 domain.
Emptage RP, Schoenberger MJ, Ferguson KM, Marmorstein R
J Biol Chem 292 19024-19033 (2017)
PMID: 28972186

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-127093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Chk1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14757 (Residues: 377-476; Coverage: 21%)
Gene names: CHEK1, CHK1

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3
Unit cell:
a: 76.818Å b: 76.818Å c: 31.828Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.244
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.
Pemberton et al. (2019)
1 more

5 mentions without citation

The PCNA interaction motifs revisited: thinking outside the PIP-box.
Prestel et al. (2019)
4 more