› 5wim

X-ray diffraction
2.55Å resolution

JAK2 Pseudokinase in complex with AT9283

Released:
DOI: 10.2210/pdb5wim/pdb
PDB entry 5wim coloured by chain, front view.
PDB entry 5wim coloured by chain, side view.
PDB entry 5wim coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery and Structural Characterization of ATP-Site Ligands for the Wild-Type and V617F Mutant JAK2 Pseudokinase Domain.
McNally R, Li Q, Li K, Dekker C, Vangrevelinghe E, Jones M, Chène P, Machauer R, Radimerski T, Eck MJ
ACS Chem Biol 14 587-593 (2019)
PMID: 30763067

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK2 Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60674 (Residues: 536-812; Coverage: 25%)
Gene name: JAK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 35R 1 x 35R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 57.791Å b: 60.687Å c: 89.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.258
Expression system: Trichoplusia ni

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Citations

5 review citations

There's more to death than life: Noncatalytic functions in kinase and pseudokinase signaling.
Mace et al. (2021)
4 more