› 5wj7

X-ray diffraction
2.5Å resolution

Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4

Released:
DOI: 10.2210/pdb5wj7/pdb
PDB entry 5wj7 coloured by chain, front view.
PDB entry 5wj7 coloured by chain, side view.
PDB entry 5wj7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod.
Andreas MP, Ajay G, Gellings JA, Rayment I
J Struct Biol 200 219-228 (2017)
PMID: 28743637

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein XRCC4; Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 22.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13426 (Residues: 2-132; Coverage: 39%)
  • Canonical: P12883 (Residues: 1733-1797; Coverage: 3%)
Gene names: MYH7, MYHCB, XRCC4
Sequence domains: DNA double-strand break repair and V(D)J recombination protein XRCC4
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 44.032Å b: 71.506Å c: 173.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot et al. (2021)
2 more

1 mention without citation

Mutations in the tail domain of MYH3 contributes to atrial septal defect.
Maran et al. (2020)