› 5wlq

X-ray diffraction
3.1Å resolution

Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1

Released:
DOI: 10.2210/pdb5wlq/pdb
PDB entry 5wlq coloured by chain, front view.
PDB entry 5wlq coloured by chain, side view.
PDB entry 5wlq coloured by chain, top view.
Source organisms:
Primary publication:
Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod.
Andreas MP, Ajay G, Gellings JA, Rayment I
J Struct Biol 200 219-228 (2017)
PMID: 28743637

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid assembly scaffolding protein; Microtubule-associated protein RP/EB family member 1; Myosin-7 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.78 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P13848 (Residues: 2-48; Coverage: 48%)
  • Canonical: P12883 (Residues: 1677-1755; Coverage: 4%)
  • Canonical: Q15691 (Residues: 208-257; Coverage: 19%)
Gene names: 7, MAPRE1, MYH7, MYHCB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand TMO 4 x TMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 141.756Å b: 141.756Å c: 87.381Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.236
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot et al. (2021)
2 more

1 mention without citation

Lack of evidence of significant homology of SARS-CoV-2 spike sequences to myocarditis-associated antigens.
Marrama et al. (2022)