› 5woh

X-ray diffraction
1.58Å resolution

Human Hemoglobin Immersed in Liquid Oxygen for 20 seconds

Released:
DOI: 10.2210/pdb5woh/pdb
PDB entry 5woh coloured by chain, front view.
PDB entry 5woh coloured by chain, side view.
PDB entry 5woh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hemoglobin crystals immersed in liquid oxygen reveal diffusion channels.
Terrell JR, Gumpper RH, Luo M
Biochem Biophys Res Commun 495 1858-1863 (2018)
PMID: 29246762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 137 amino acids
Theoretical weight: 14.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 3-139; Coverage: 97%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 75.43Å b: 83.12Å c: 83.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.207

Quick links

Citations

4 citation in other articles

Metformin Affects Heme Function as a Possible Mechanism of Action.
Li et al. (2019)
3 more

2 mentions without citation

Hemoglobin is an oxygen-dependent glutathione buffer adapting the intracellular reduced glutathione levels to oxygen availability.
Fenk et al. (2022)
1 more