› 5wp3

X-ray diffraction
2.55Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131023 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 42.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75530 (Residues: 75-441; Coverage: 83%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
EB22 Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.99 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ribosome-recycling factor

Ligands and Environments

1 bound ligand:
Ligand UNX 16 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 57.07Å b: 134.29Å c: 61.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Mechanisms of gene regulation in human embryos and pluripotent stem cells.
Theunissen et al. (2017)
6 more

1 mention without citation

First critical repressive H3K27me3 marks in embryonic stem cells identified using designed protein inhibitor.
Moody et al. (2017)