› 5wpz

X-ray diffraction
2Å resolution

Crystal structure of MNDA PYD with MBP tag

Released:
DOI: 10.2210/pdb5wpz/pdb
PDB entry 5wpz coloured by chain, front view.
PDB entry 5wpz coloured by chain, side view.
PDB entry 5wpz coloured by chain, top view.
Source organisms:
Primary publication:
Design of an expression system to enhance MBP-mediated crystallization.
Jin T, Chuenchor W, Jiang J, Cheng J, Li Y, Fang K, Huang M, Smith P, Xiao TS
Sci Rep 7 40991 (2017)
PMID: 28112203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Myeloid cell nuclear differentiation antigen Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 477 amino acids
Theoretical weight: 52.8 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
  • Canonical: P41218 (Residues: 4-98; Coverage: 23%)
Gene names: JW3994, MNDA, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 192.15Å b: 235.61Å c: 73.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.269
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Polyionic Tags as Enhancers of Protein Solubility in Recombinant Protein Expression.
Paraskevopoulou et al. (2018)
1 more

1 mention without citation

Activation and Immune Regulation Mechanisms of PYHIN Family During Microbial Infection.
Fan et al. (2021)