› 5wqt

X-ray diffraction
2.64Å resolution

Structure of a protein involved in pyroptosis

Released:
DOI: 10.2210/pdb5wqt/pdb
PDB entry 5wqt coloured by chain, front view.
PDB entry 5wqt coloured by chain, side view.
PDB entry 5wqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure insight of GSDMD reveals the basis of GSDMD autoinhibition in cell pyroptosis.
Kuang S, Zheng J, Yang H, Li S, Duan S, Shen Y, Ji C, Gan J, Xu XW, Li J
Proc Natl Acad Sci U S A 114 10642-10647 (2017)
PMID: 28928145

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gasdermin-D, C-terminal Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P57764 (Residues: 276-484; Coverage: 43%)
Gene names: DFNA5L, FKSG10, GSDMD, GSDMDC1
Sequence domains: Gasdermin PUB domain

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand CIT 2 x CIT
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 47.886Å b: 86.165Å c: 112.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.235 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

50 review citations

Pyroptosis: mechanisms and diseases.
Yu et al. (2021)
49 more

4 mentions without citation

Structure insight of GSDMD reveals the basis of GSDMD autoinhibition in cell pyroptosis.
Kuang et al. (2017)
3 more