› 5wtb

X-ray diffraction
3.3Å resolution

Complex Structure of Staphylococcus aureus SdrE with human complement factor H

Released:
DOI: 10.2210/pdb5wtb/pdb
PDB entry 5wtb coloured by chain, front view.
PDB entry 5wtb coloured by chain, side view.
PDB entry 5wtb coloured by chain, top view.
Source organisms:
Primary publication:
Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion.
Zhang Y, Wu M, Hang T, Wang C, Yang Y, Pan W, Zang J, Zhang M, Zhang X
Biochem J 474 1619-1631 (2017)
PMID: 28258151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140217 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine-aspartate repeat-containing protein E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 338 amino acids
Theoretical weight: 37.69 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q932F7 (Residues: 270-599; Coverage: 30%)
Gene names: SAV0563, sdrE
Sequence domains:
Structure domains:
Complement factor H Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 21 amino acids
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P08603 (Residues: 1206-1226; Coverage: 2%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 117.5Å b: 117.5Å c: 154.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Pathogenicity and virulence of Staphylococcus aureus.
Cheung et al. (2021)
9 more

4 mentions without citation

Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion.
Zhang et al. (2017)
3 more