› 5wum

X-ray diffraction
2Å resolution

Crystal structure of mouse importin-alpha1 bound to S385-phosphorylated NLS of EBNA1

Released:
DOI: 10.2210/pdb5wum/pdb
PDB entry 5wum coloured by chain, front view.
PDB entry 5wum coloured by chain, side view.
PDB entry 5wum coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the regulation of nuclear import of Epstein-Barr virus nuclear antigen 1 (EBNA1) by phosphorylation of the nuclear localization signal.
Nakada R, Hirano H, Matsuura Y
Biochem Biophys Res Commun 484 113-117 (2017)
PMID: 28104399

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136655 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 49.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Epstein-Barr nuclear antigen 1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 9 amino acids
Theoretical weight: 1.13 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03211 (Residues: 378-386; Coverage: 1%)
Gene names: BKRF1, EBNA1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 77.91Å b: 90.3Å c: 99.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

EBNA1-targeted inhibitors: Novel approaches for the treatment of Epstein-Barr virus-associated cancers.
Jiang et al. (2018)
1 more

1 mention without citation

Crystal structure of importin-α bound to the nuclear localization signal of Epstein-Barr virus EBNA-LP protein.
Nakada et al. (2017)