› 5wy0

X-ray diffraction
2Å resolution

Crystal structure of the methyltranferase domain of human HEN1 in complex with AdoMet

Released:
DOI: 10.2210/pdb5wy0/pdb
PDB entry 5wy0 coloured by chain, front view.
PDB entry 5wy0 coloured by chain, side view.
PDB entry 5wy0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
Peng L, Zhang F, Shang R, Wang X, Chen J, Chou JJ, Ma J, Wu L, Huang Y
J Biol Chem 293 9981-9994 (2018)
PMID: 29703750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small RNA 2'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T8I9 (Residues: 31-258; Coverage: 58%)
Gene names: C1orf59, HENMT1
Sequence domains: Methyltransferase domain

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 38.521Å b: 38.521Å c: 249.437Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.204 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

PIWI Proteins and piRNAs in the Nervous System.
Kim KW. (2019)
1 more

2 mentions without citation

Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
Peng et al. (2018)
1 more