› 5wzz

X-ray diffraction
2.1Å resolution

The SIAH E3 ubiquitin ligases promote Wnt/ beta-catenin signaling through mediating Wnt-induced Axin degradation

Released:
DOI: 10.2210/pdb5wzz/pdb
PDB entry 5wzz coloured by chain, front view.
PDB entry 5wzz coloured by chain, side view.
PDB entry 5wzz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The SIAH E3 ubiquitin ligases promote Wnt/β-catenin signaling through mediating Wnt-induced Axin degradation.
Ji L, Jiang B, Jiang X, Charlat O, Chen A, Mickanin C, Bauer A, Xu W, Yan X, Cong F
Genes Dev 31 904-915 (2017)
PMID: 28546513

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127344 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 21.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q8IUQ4 (Residues: 93-282; Coverage: 67%)
Gene names: HUMSIAH, SIAH1
Sequence domains:
Structure domains:
Axin-1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 20 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O15169 (Residues: 375-394; Coverage: 2%)
Gene names: AXIN, AXIN1

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 41.502Å b: 86.45Å c: 117.454Å
α: 90° β: 98.43° γ: 90°
R-values:
R R work R free
0.186 0.184 0.208
Expression system: Escherichia coli K-12

Quick links

Citations

13 review citations

The role of ubiquitination in tumorigenesis and targeted drug discovery.
Deng et al. (2020)
12 more

1 mention without citation

The SIAH E3 ubiquitin ligases promote Wnt/β-catenin signaling through mediating Wnt-induced Axin degradation.
Ji et al. (2017)