› 5x8l

X-ray diffraction
3.1Å resolution

PD-L1 in complex with atezolizumab

Released:
DOI: 10.2210/pdb5x8l/pdb
PDB entry 5x8l coloured by chain, front view.
PDB entry 5x8l coloured by chain, side view.
PDB entry 5x8l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of PD-1/PD-L1 blockade via anti-PD-L1 antibodies atezolizumab and durvalumab.
Lee HT, Lee JY, Lim H, Lee SH, Moon YJ, Pyo HJ, Ryu SE, Shin W, Heo YS
Sci Rep 7 5532 (2017)
PMID: 28717238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212801 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Programmed cell death 1 ligand 1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 123 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9NZQ7 (Residues: 18-134; Coverage: 43%)
Gene names: B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
atezolizumab light chain Chains: K, L, M, N, O
Molecule details ›
Chains: K, L, M, N, O
Length: 214 amino acids
Theoretical weight: 23.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Structure domains: Immunoglobulins
atezolizumab heavy chain Chains: F, G, H, J, S
Molecule details ›
Chains: F, G, H, J, S
Length: 230 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 92.197Å b: 169.793Å c: 206.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.256
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

39 review citations

First line Immunotherapy for Non-Small Cell Lung Cancer.
Nasser et al. (2020)
38 more

14 mentions without citation

Molecular Interactions of Antibody Drugs Targeting PD-1, PD-L1, and CTLA-4 in Immuno-Oncology.
Lee et al. (2019)
13 more