› 5x8q

X-ray diffraction
2.2Å resolution

Crystal Structure of the mutant Human ROR gamma Ligand Binding Domain With rockogenin.

Released:
DOI: 10.2210/pdb5x8q/pdb
PDB entry 5x8q coloured by chain, front view.
PDB entry 5x8q coloured by chain, side view.
PDB entry 5x8q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ternary complex of human RORγ ligand-binding domain, inverse agonist and SMRT peptide shows a unique mechanism of corepressor recruitment.
Noguchi M, Nomura A, Murase K, Doi S, Yamaguchi K, Hirata K, Shiozaki M, Hirashima S, Kotoku M, Yamaguchi T, Katsuda Y, Steensma R, Li X, Tao H, Tse B, Fenn M, Babine R, Bradley E, Crowe P, Thacher S, Adachi T, Kamada M
Genes Cells 22 535-551 (2017)
PMID: 28493531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear receptor ROR-gamma Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 258 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51449 (Residues: 261-518; Coverage: 50%)
Gene names: NR1F3, RORC, RORG, RZRG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor corepressor 2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 22 amino acids
Theoretical weight: 2.6 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: Q9Y618 (Residues: 2335-2356; Coverage: 1%)
Gene names: CTG26, NCOR2

Ligands and Environments

1 bound ligand:
Ligand 82R 4 x 82R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 79.63Å b: 71.94Å c: 99.21Å
α: 90° β: 90.06° γ: 90°
R-values:
R R work R free
0.193 0.191 0.241
Expression systems:
  • Escherichia coli
  • synthetic construct

Quick links

Citations

5 citation in other articles

A molecular switch regulating transcriptional repression and activation of PPARγ.
Shang et al. (2020)
4 more

3 mentions without citation

RORγ Structural Plasticity and Druggability.
Huang et al. (2020)
2 more