› 5xby

X-ray diffraction
3.25Å resolution

Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)

Released:
DOI: 10.2210/pdb5xby/pdb
PDB entry 5xby coloured by chain, front view.
PDB entry 5xby coloured by chain, side view.
PDB entry 5xby coloured by chain, top view.
Source organisms:
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A; cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 11.13 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P13861 (Residues: 5-44; Coverage: 10%)
  • Canonical: P38507 (Residues: 217-269; Coverage: 11%)
Gene names: PKR2, PRKAR2, PRKAR2A, spa
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2221
Unit cell:
a: 75.988Å b: 174.147Å c: 103.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.251 0.307
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles