› 5xhe

X-ray diffraction
1.4Å resolution

Crystal structure analysis of the second bromodomain of BRD2 covalently linked to b-mercaptoethanol

Released:
DOI: 10.2210/pdb5xhe/pdb
PDB entry 5xhe coloured by chain, front view.
PDB entry 5xhe coloured by chain, side view.
PDB entry 5xhe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the crystal structure of BRD2-BD2 - phenanthridinone complex and theoretical studies on phenanthridinone analogs.
Mathur S, Deshmukh P, Tripathi S, Marimuthu P, Padmanabhan B
J Biomol Struct Dyn 36 2342-2360 (2018)
PMID: 28696179

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 2 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 13.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25440 (Residues: 348-455; Coverage: 14%)
Gene names: BRD2, KIAA9001, RING3
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

2 bound ligands:
Ligand PGE 1 x PGE
Ligand GOL 3 x GOL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Unit cell:
a: 32.19Å b: 52.45Å c: 71.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1.
Chavez et al. (2021)
3 more