5xj3

X-ray diffraction
3.2Å resolution

Complex structure of ipilimumab-scFv and CTLA-4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-147702 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ipilimumab-VH Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
ipilimumab-VL Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 109 amino acids
Theoretical weight: 11.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Cytotoxic T-lymphocyte protein 4 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 127 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16410 (Residues: 36-161; Coverage: 67%)
Gene names: CD152, CTLA4
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Unit cell:
a: 91.959Å b: 114.24Å c: 150.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.264
Expression system: Escherichia coli