› 5xja

X-ray diffraction
3.43Å resolution

The Crystal Structure of the Minimal Core Domain of the Microtubule Depolymerizer KIF2C Complexed with ADP-Mg-AlFx

Released:
DOI: 10.2210/pdb5xja/pdb
PDB entry 5xja coloured by chain, front view.
PDB entry 5xja coloured by chain, side view.
PDB entry 5xja coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mechanism of Catalytic Microtubule Depolymerization via KIF2-Tubulin Transitional Conformation.
Ogawa T, Saijo S, Shimizu N, Jiang X, Hirokawa N
Cell Rep 20 2626-2638 (2017)
PMID: 28903043

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF2C Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 48.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q922S8 (Residues: 183-585; Coverage: 56%)
Gene name: Kif2c
Sequence domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
Ligand AF3 2 x AF3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 91.098Å b: 166.989Å c: 74.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.23
Expression system: Escherichia coli

Quick links

Citations

6 review citations

These motors were made for walking.
Hunter et al. (2020)
5 more

2 mentions without citation

Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.
Benoit et al. (2018)
1 more