› 5xjm

X-ray diffraction
3.2Å resolution

Complex structure of angiotensin II type 2 receptor with Fab

Released:
DOI: 10.2210/pdb5xjm/pdb
PDB entry 5xjm coloured by chain, front view.
PDB entry 5xjm coloured by chain, side view.
PDB entry 5xjm coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the human angiotensin II type 2 receptor bound to an angiotensin II analog.
Asada H, Horita S, Hirata K, Shiroishi M, Shiimura Y, Iwanari H, Hamakubo T, Shimamura T, Nomura N, Kusano-Arai O, Uemura T, Suno C, Kobayashi T, Iwata S
Nat Struct Mol Biol 25 570-576 (2018)
PMID: 29967536

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208325 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Soluble cytochrome b562; Type-2 angiotensin II receptor Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 48.06 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50052 (Residues: 35-242, 246-346; Coverage: 85%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: AGTR2, cybC
Sequence domains:
FabH Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
FabL Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Angiotensin-4 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 970 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01019 (Residues: 26-31; Coverage: 1%)
Gene names: AGT, SERPINA8

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SAR 1 x SAR

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21212
Unit cell:
a: 465.39Å b: 48.65Å c: 55.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.275
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus
  • Not provided

Quick links

Citations

3 review citations

The Angiotensin AT2 Receptor: From a Binding Site to a Novel Therapeutic Target.
Steckelings et al. (2022)
2 more

22 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
21 more