› 5xk4

Solution NMR

Retracted state of S65-phosphorylated ubiquitin

Released:
DOI: 10.2210/pdb5xk4/pdb
PDB entry 5xk4 coloured by chain, ensemble of 30 models, front view.
PDB entry 5xk4 coloured by chain, ensemble of 30 models, side view.
PDB entry 5xk4 coloured by chain, ensemble of 30 models, top view.
Source organism: Homo sapiens
Primary publication:
Ubiquitin S65 phosphorylation engenders a pH-sensitive conformational switch.
Dong X, Gong Z, Lu YB, Liu K, Qin LY, Ran ML, Zhang CL, Liu Z, Zhang WP, Tang C
Proc Natl Acad Sci U S A 114 6770-6775 (2017)
PMID: 28611216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 71%
Refinement method: simulated annealing
Expression system: Escherichia coli K-12

Quick links

Citations

2 review citations

On the necessity of an integrative approach to understand protein structural dynamics.
Yang et al. (2019)
1 more

3 mentions without citation

An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1.
Gladkova et al. (2017)
2 more