› 5xod

X-ray diffraction
1.85Å resolution

Crystal structure of human Smad2-Ski complex

Released:
DOI: 10.2210/pdb5xod/pdb
PDB entry 5xod coloured by chain, front view.
PDB entry 5xod coloured by chain, side view.
PDB entry 5xod coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hydrophobic patches on SMAD2 and SMAD3 determine selective binding to cofactors.
Miyazono KI, Moriwaki S, Ito T, Kurisaki A, Asashima M, Tanokura M
Sci Signal 11 (2018)
PMID: 29588413

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146310 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 2 Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15796 (Residues: 262-458; Coverage: 42%)
Gene names: MADH2, MADR2, SMAD2
Sequence domains: MH2 domain
Structure domains: Tumour Suppressor Smad4
Ski oncogene Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12755 (Residues: 15-40; Coverage: 4%)
Gene name: SKI

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: I23
Unit cell:
a: 110.84Å b: 110.84Å c: 110.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.254
Expression system: Escherichia coli

Quick links

Citations

2 review citations

TGFβ-Directed Therapeutics: 2020.
Teicher BA. (2021)
1 more

1 mention without citation

Structural basis for receptor-regulated SMAD recognition by MAN1.
Miyazono et al. (2018)