5xp1

X-ray diffraction
2.88Å resolution

Structure of monomeric mutant of REI immunoglobulin light chain variable domain crystallized at pH 6

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin kappa variable 1D-33 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 109 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01593 (Residues: 23-117; Coverage: 100%)
Gene name: IGKV1D-33
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P4
Unit cell:
a: 130.76Å b: 130.76Å c: 92.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.257 0.297
Expression system: Escherichia coli BL21(DE3)