› 5xpt

X-ray diffraction
2.1Å resolution

Crystal structure of MAD2L2/REV7 in complex with a CAMP fragment in a tetragonal crystal

Released:
DOI: 10.2210/pdb5xpt/pdb
PDB entry 5xpt coloured by chain, front view.
PDB entry 5xpt coloured by chain, side view.
PDB entry 5xpt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dynamic feature of mitotic arrest deficient 2-like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment-maintaining phosphoprotein (CAMP).
Hara K, Taharazako S, Ikeda M, Fujita H, Mikami Y, Kikuchi S, Hishiki A, Yokoyama H, Ishikawa Y, Kanno SI, Tanaka K, Hashimoto H
J Biol Chem 292 17658-17667 (2017)
PMID: 28887307

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188579 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 26.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI95 (Residues: 1-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Structure domains: HORMA domain
Chromosome alignment-maintaining phosphoprotein 1 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96JM3 (Residues: 325-344; Coverage: 3%)
Gene names: C13orf8, CAMP, CHAMP, CHAMP1, KIAA1802, ZNF828

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I422
Unit cell:
a: 106.493Å b: 106.493Å c: 127.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

DNA polymerase ΞΆ in DNA replication and repair.
Martin et al. (2019)
6 more

7 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
6 more