› 5xtz

X-ray diffraction
2.1Å resolution

Crystal structure of GAS41 YEATS bound to H3K27ac peptide

Released:
DOI: 10.2210/pdb5xtz/pdb
PDB entry 5xtz coloured by chain, front view.
PDB entry 5xtz coloured by chain, side view.
PDB entry 5xtz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Gas41 links histone acetylation to H2A.Z deposition and maintenance of embryonic stem cell identity.
Hsu CC, Zhao D, Shi J, Peng D, Guan H, Li Y, Huang Y, Wen H, Li W, Li H, Shi X
Cell Discov 4 28 (2018)
PMID: 29900004

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131955 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
YEATS domain-containing protein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 18.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O95619 (Residues: 15-159; Coverage: 64%)
Gene names: GAS41, YEATS4
Sequence domains: YEATS family
Structure domains: YEATS domain
Histone H3.1 Chain: E
Molecule details ›
Chain: E
Length: 10 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 23-32; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 75.864Å b: 80.663Å c: 107.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

9 review citations

The histone variant H2A.Z in gene regulation.
Giaimo et al. (2019)
8 more

3 mentions without citation

Gas41 links histone acetylation to H2A.Z deposition and maintenance of embryonic stem cell identity.
Hsu et al. (2018)
2 more