› 5y2s

X-ray diffraction
0.9Å resolution

7.0 atm CO2-pressurized human carbonic anhydrase II

Released:
DOI: 10.2210/pdb5y2s/pdb
PDB entry 5y2s coloured by chain, front view.
PDB entry 5y2s coloured by chain, side view.
PDB entry 5y2s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Active-site solvent replenishment observed during human carbonic anhydrase II catalysis.
Kim JK, Lomelino CL, Avvaru BS, Mahon BP, McKenna R, Park S, Kim CU
IUCrJ 5 93-102 (2018)
PMID: 29354275

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CO2 2 x CO2
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 42.305Å b: 41.365Å c: 71.944Å
α: 90° β: 104.12° γ: 90°
R-values:
R R work R free
0.112 0.112 0.127
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Crystallography and Its Impact on Carbonic Anhydrase Research.
Lomelino et al. (2018)
1 more

2 mentions without citation

Active-site solvent replenishment observed during human carbonic anhydrase II catalysis.
Kim et al. (2018)
1 more