› 5y7n

X-ray diffraction
2.5Å resolution

Crystal structure of AKR1B10 in complex with NADP+ and Androst-4-ene-3-beta-6-alpha-diol

Released:
DOI: 10.2210/pdb5y7n/pdb
PDB entry 5y7n coloured by chain, front view.
PDB entry 5y7n coloured by chain, side view.
PDB entry 5y7n coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Hong Z, Liping Z, Cuiyun L, Wei Z

Function and Biology Details

Reactions catalysed: 
All-trans-retinol + NADP(+) = all-trans-retinal + NADPH
Allyl alcohol + NADP(+) = acrolein + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo-keto reductase family 1 member B10 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60218 (Residues: 1-316; Coverage: 100%)
Gene names: AKR1B10, AKR1B11
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand 8QL 1 x 8QL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P61
Unit cell:
a: 89.864Å b: 89.864Å c: 78.309Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.245
Expression system: Escherichia coli

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