› 5y80

X-ray diffraction
2.5Å resolution

Complex structure of cyclin G-associated kinase with gefitinib

Released:
DOI: 10.2210/pdb5y80/pdb
PDB entry 5y80 coloured by chain, front view.
PDB entry 5y80 coloured by chain, side view.
PDB entry 5y80 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for the Inhibition of Cyclin G-Associated Kinase by Gefitinib.
Ohbayashi N, Murayama K, Kato-Murayama M, Kukimoto-Niino M, Uejima T, Matsuda T, Ohsawa N, Yokoyoma S, Nojima H, Shirouzu M
ChemistryOpen 7 721-727 (2018)
PMID: 30214852

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208048 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-G-associated kinase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14976 (Residues: 25-335; Coverage: 24%)
Gene names: DNAJC26, GAK
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1
NANOBODY Chain: B
Molecule details ›
Chain: B
Length: 148 amino acids
Theoretical weight: 16.19 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand IRE 2 x IRE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 70.331Å b: 73.87Å c: 95.311Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.282
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Towards the Development of an In vivo Chemical Probe for Cyclin G Associated Kinase (GAK).
Asquith et al. (2019)
2 more

3 mentions without citation

COVID-19: Drug Targets and Potential Treatments.
Gil et al. (2020)
2 more