› 5y9j

X-ray diffraction
2.05Å resolution

BAFF in complex with belimumab

Released:
DOI: 10.2210/pdb5y9j/pdb
PDB entry 5y9j coloured by chain, front view.
PDB entry 5y9j coloured by chain, side view.
PDB entry 5y9j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BAFF-neutralizing interaction of belimumab related to its therapeutic efficacy for treating systemic lupus erythematosus.
Shin W, Lee HT, Lim H, Lee SH, Son JY, Lee JU, Yoo KY, Ryu SE, Rhie J, Lee JY, Heo YS
Nat Commun 9 1200 (2018)
PMID: 29572471

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212868 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
belibumab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
belimumab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 24.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Tumor necrosis factor ligand superfamily member 13b, soluble form Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y275 (Residues: 134-285; Coverage: 53%)
Gene names: BAFF, BLYS, TALL1, TNFSF13B, TNFSF20, UNQ401/PRO738, ZTNF4
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P213
Unit cell:
a: 134.001Å b: 134.001Å c: 134.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli

Quick links

Citations

2 review citations

BAFF inhibition in SLE-Is tolerance restored?
Jackson et al. (2019)
1 more

4 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
3 more