› 5yc2

X-ray diffraction
2.7Å resolution

Crystal structure of inner membrane protein Bqt4 in complex with telomeric protein Rap1

Released:
DOI: 10.2210/pdb5yc2/pdb
PDB entry 5yc2 coloured by chain, front view.
PDB entry 5yc2 coloured by chain, side view.
PDB entry 5yc2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.
Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y
Nucleic Acids Res 47 1573-1584 (2019)
PMID: 30462301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129903 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bouquet formation protein 4; Ubiquitin-like protein SMT3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 207 amino acids
Theoretical weight: 23.57 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12306 (Residues: 20-93; Coverage: 73%)
  • Canonical: O60158 (Residues: 9-140; Coverage: 31%)
Gene names: D9719.15, SMT3, SPBC19C7.10, YDR510W, bqt4
Sequence domains: Ubiquitin family
DNA-binding protein rap1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 18 amino acids
Theoretical weight: 2 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96TL7 (Residues: 498-512; Coverage: 2%)
Gene names: SPBC1778.02, rap1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 32.154Å b: 51.293Å c: 79.669Å
α: 94.92° β: 100.56° γ: 105°
R-values:
R R work R free
0.204 0.202 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast.
Hirano et al. (2020)
1 more

1 mention without citation

Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.
Hu et al. (2019)