5yc3

X-ray diffraction
2.6Å resolution

Crystal structure of AL3 PHD finger bound to H3K4me2

Released:
Source organism: Arabidopsis thaliana
Primary publication:
Systematic Profiling of Histone Readers in Arabidopsis thaliana.
Cell Rep 22 1090-1102 (2018)
PMID: 29386129

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-157788 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PHD finger protein ALFIN-LIKE 3 Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 6.53 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9M2B4 (Residues: 191-245; Coverage: 22%)
Gene names: AL3, At3g42790, T21C14_10
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: P59226 (Residues: 2-10; Coverage: 7%)
Gene names: At1g09200, At3g27360, At5g10390, At5g10400, At5g65360, F12B17_250, F12B17_260, HTR1, HTR13, HTR2, HTR3, HTR9, K1G2.8, MNA5.9, T12M4.9

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P4122
Unit cell:
a: 30.921Å b: 30.921Å c: 157.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.191 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided