5yi8

X-ray diffraction
2Å resolution

Crystal structure of drosophila Numb PTB domain and Pon peptide complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-147762 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein numb Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16554 (Residues: 64-203; Coverage: 25%)
Gene names: CG3779, numb
Sequence domains: Phosphotyrosine interaction domain (PTB/PID)
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Partner of numb Chain: B
Molecule details ›
Chain: B
Length: 33 amino acids
Theoretical weight: 3.91 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: Q9W4I7 (Residues: 128-160; Coverage: 5%)
Gene names: CG3346, Dmel\CG3346, Dmel_CG3346, PON, Pon, pon

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 43.392Å b: 43.392Å c: 181.304Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.227
Expression systems:
  • Escherichia coli BL21
  • Not provided